Molecular Services

Next-Generation Sequencing

NGS banner

At AITbiotech, we are proud to offer the broadest options in Next Generation Sequencing (NGS) services using the latest technologies in the market. Our NGS services are available on leading sequencing platforms, Illumina (HiSeq and MiSeq) and Life Technologies (Ion Proton and Ion PGM), and we have extensive expertise using these platforms in a wide array of NGS sequencing projects.

Fully supported by a team of technical staff and scientists experienced in this fast evolving technology, we will assist you in selecting the best sequencing platform and solutions for your specific needs. Leverage on our expertise and contact us today for a free, no-obligation consultation.

Features

  • Comprehensive NGS solutions, from sample preparation to bioinformatics analysis
  • Rigorous quality control in every step of the NGS workflow
  • Fast turnaround time
  • Experienced technical and scientific support at your service
  • Personalized NGS services based on project-specific needs available

Types of Bioinformatic Services Offered

  • De novo genome assembly
  • Reference mapping
  • Variant analysis
  • Differential gene expression analysis
  • Copy number and structural variation analysis
  • Custom pipeline development, visualization and reporting
  • 16S rRNA sequencing and species identification
  • Methylation-Seq, whole epigenome, reduced dataset epigenomics

Types of Next Generation Sequencing Services Offered

Type Description
Whole genome sequencing Determines the complete DNA sequence of an organism’s genome at a single time
Targeted sequencing Focus on specific areas of interest within the genome
RNA-seq Quantification technique to quantify the expression levels of each transcript during development or under different physiologic and pathological conditions
Metagenomics Microbial genomes, viral genomes, human genome
Epigenomics Custom and commercial amplicon panels and sequencing, whole and reduced exomeComplete transcriptome, differential gene expression, small RNA and non-coding RNA sequencing, targeted expressed RNA sequencing

 

Testimonials

“We are pleased with the services and results provided by AITbiotech. Their team is knowledgeable and responsive to our needs and queries. We have had experience with other suppliers of NGS services and we find that AITbiotech provides more efficient and reliable services, with more stringent QC that ensures higher quality NGS reads. Working with AITbiotech has the additional advantage that it is not an agent for another company located elsewhere. Instead the data are generated by AITbiotech personnel in Singapore which helps with communicating with the NGS lab and avoids the hidden costs and bureaucratic hurdles that come with international shipping of samples. These positive experiences mean that we will continue to work with AITbiotech for all our next-generation sequencing needs.”~Professor Rudolf Meier, Department of Biological Sciences, National University of Singapore


“I am an animal physiologist and a Professorin the Department of Biological Sciences in the National University of Singapore. My students and I have engaged AITbiotech’s NGS service to investigate the up- and down-regulation of gene expression in several fish species in response to various environmental conditions using the Illumina platform. We have also requested AITbiotech to provide us with the bioinformatics service required for de novo assembly and the generation of differential libraries using Illumina reads. In addition, AITbiotech has helped us examine nucleotide substitutions in the cDNA sequence of a specific gene using the Ion Torrent platform with the necessary bioinformatics support. So far, I have very good experience with AITbiotech’s NGS service. The AITbiotech NGS application and products specialist very knowledgeable and can provide useful suggestions/advices where applicable and when needed. The NGS, in particular Ion Torrent, running time is short and reasonable. To cater for meaningful bioinformatics analysis, the AITbiotech bioinformatic specialist works closely with the customer to resolve technical issues/problems. The NGS data obtained through AITbiotech’s service have effectively speed up the research progress in my laboratory, and provided my student with the opportunity to discover novel genes and/or gene functions.”

~Professor Alex Y.K. Ip, Department of Biological Sciences, National University of Singapore