Molecular Services

DNA Sequencing

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DNA Sequencing at AITbiotech is performed following ISO 13485 quality standards, ensuring a high quality of capillary electrophoresis sequencing for Singapore and SEA region customers.

All DNA samples are sequenced using BigDye Terminator chemistry with available universal primers or user-supplied primers. The DNA sequencing reactions are electrophoresed on ABI’s 3730XL DNA Analyzers which can produce read lengths of 800-1000 bases.

Sequencing results undergo stringent quality controls, which ensures high quality sequences for downstream applications.

Purchase AITbiotech Sequencing Package to get the best value for your sequences!

Features

  • 24 – 48 hr turnaround time (working days)
  • Easy and flexible submission requirements – No DNA and primer premixing. No special sample tubes required.
  • Incoming samples are always quality checked using gel electrophoresis for PCR products and spectrophotometry for plasmids
  • Reproducible analysis
  • Free universal primers are provided for sequencing runs (AITBiotech Universal Primers List)
  • Template preparation and primer synthesis services are available
  • Technical and scientific support by experienced professionals

Types of Capillary DNA Sequencing Services Offered

Type Description
Standard Sequencing Include cycle sequencing reactions, dye terminator removal, capillary electrophoresis run and result analysis.
Ready-to-run Sequencing Include capillary electrophoresis run and result analysis only.
Ready-to-run Sequencing with Dye Removal Include dye terminator removal, capillary electrophoresis run and result analysis.
PCR Clean-up Sequencing Include unpurified PCR template clean-up by either gel or column methods, followed by Standard Sequencing service.
Clone Sequencing Include single colony growth, plasmid template extraction and purification, followed by Standard Sequencing service.
Primer Walking Sequencing Include subsequent primer design in one or both directional to fully sequence the region of interest in the plasmid.

 

Refer to our FAQ section for more details!