Instrument

abCylerQ

Real-time PCR System

The abCyclerQ is a powerful tool used to perform rapid and precise real-time polymerase chain reaction (RT-PCR). This six-channel (5 fluorescence colours and 1 FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver reliable detection for both single and multiplex real-time PCR assays.

Your preferred choice for qPCR

Features

  • 10 inch touch screen monitor
  • Easy-to-use software
  • LED excitation source and PD detection
  • Special temperature protocol advantage
  • 7 seconds top optical scanning
  • Outstanding and powerful data analysis function

Product Details

TECHNICAL SPECIFICATIONS

 Model  abCyclerQ
 Catalogue no.  800700
 Sample capacity (wells)
  • 12 x 0.2ml 8-tube qPCR strips/ 96 x 0.2ml qPCR tubes
  • 1 qPCR plate 8×12 (No skirt)
 Tube type  Clear, White, Frosted
 Thermoblock  Aluminium
 Reaction volume  Up to 100µl
 Temperature range  0-100°C
 Heating technology of the block  Peltier element
 Max. heating rate  6.1°C/sec
 Average heating rate  4.5°C/sec
 Max. cooling rate  5.0°C/sec
 Average cooling rate  2.8°C/sec
 Temperature accuracy  ± 0.2°C
 Block uniformity
20- 72°C
95°C
± 0.2°C
± 0.3°C
 Gradient range  1- 40°C
 Gradient block  12 columns
 Gradient temperature range  35- 100°C
 Lid temperature range (Heated lid)
  • Enabled: 40- 110°C
  • Disabled: Room temperature to 110°C
 Special temperature protocol  Standard mode, Gradient PCR mode, Long PCR mode, Touchdown PCR mode
 Sample linearity  R2 ≥ 0.998 or r ≥ |0.999|
 Sample repeatability  Ct value, CV ≤ 0.5%
 Sample dynamic range  1- 1010 copies
 Excitation source  6 filtered LED
 Detection  Photodiodes
 Detection position  Excitation and detection from the top
 Detection method  6 channels scanning at the same time, no edge effect
 Scan time  7 seconds for 96 wells for all channels
 Channels & wavelengths
  • 465/510 (FAM, SYBR Green I, SYTO9, EvaGreen, LCGreen)
  • 527/ 563 (HEX, VIC, TET, JOE)
  • 580/ 616 (ROX, Texas Red)
  • 632/ 664 (Cy5)
  • 680/ 730 (Alexa Fluor680)
  • 465/ 616 (FRET)
 Probe  TaqMan probe, Molecular beacon probe, Scorpion probe, FRET
 Multiplexing  Up to 5 targets per well
 Fluorescence linearity  R2 > 0.980 or r > |0.990|
 Data analysis modes  Qualitative analysis, Absolute quantification, Relative quantification, Genotyping, Endpoint analysis, Melt curve analysis, High resolution melt analysis
 Control modes
  • 10.4″ touch screen
  • Direct PC control/ Standalone system
  • WLAN control (One PC can connect up to 10 units)
 Sample loading  Touch screen command on loading platform
 Data storage  Upload & download via USB; save up to 1000 run files in internal memory storage
 Power failure protection  Continue with experiments after power supply resumes
 Customize report  Default template available with customizable options
 Shipping lock  Automatically detect shipping lock upon start up
 LIS connection  CSV, Excel, TXT format data output, open port for LIS connection
 PC operating system  Win 7, Win 10
 Communication port  1 ethernet and 3 USB
 Dimension (W x D x H)  355mm x 480mm x 485mm
 Weight  30kg
 Power usage  AC100- 240V, 50/60Hz
 Power consumption  900VA
 Working environment
  • Temperature: 10-30°C
  • Humidity: 20- 85%