Oligos Design & Analysis Tools
Primer3 is a free online tool to design and analyze primers for PCR and real time PCR.
Primer-BLAST is developed at NCBI to help users make primers that are specific to the input PCR template. It uses Primer3 to design PCR primers and then submits them to BLAST search against user-selected database. The blast results will be automatically analyzed to avoid primer pairs that can cause amplification of targets other than the input template.