Molecular Diagnostics  Oligonucleotides Synthesis  Capillary Sequencing  Next-Generation Sequencing  Bioinformatics Analysis 

Since 2008, AITbiotech has provided high quality capillary electrophoresis sequencing for Singapore customers as well as SEA region customers. All DNA samples are sequenced using BigDye Terminator chemistry with available universal primers or user-supplied primers. The DNA sequencing reactions are electrophoresed on ABI’s 3730XL DNA Analyzers which can produce read lengths of 800-1000 bases. Sequencing results undergo stringent QC and only results which passed QC are released to the customers.

Features

  • 24 - 48 hr turnaround time
  • Easy & flexible submission requirements - No DNA & primer premixing. No special sample tubes required
  • Incoming samples are quality checked using gel electrophoresis for PCR products and spectrophotometry for plasmids
  • 95% accuracy up to 800bp
  • Reproducible analysis
  • Free universal primers are provided for sequencing runs (Click here for a list of free primers Sequencing Universal Primers)
  • Template preparation and primer synthesis services are available
  • Technical and scientific support by experienced professionals

 

How to order 

Capillary Sequencing Orders
(Quick Guide)

  1. Fill up the Capillary Sequencing Form (CS) Form (Please click here to download CS Form), alternatively, the CS Form can be found in the bag provided by AITbiotech
  2. Please put your template or primers in the Sample Loading Card in the bag provided by AITbiotech
  3. Submit the CS Form either by email to sequencing@aitbiotech.com or put the CS Form together with your DNA template and primer in the bag supplied by AITbiotech
  4. Contact AITbiotech for sample collection date and time either by email to sequencing@aitbiotech.com or call us at +65 6778 6822
  5. AITbiotech dispatch will collect your samples and the sequencing results will be delivered to you via email in 1-2 working days

 

Types of Capillary Sequencing (CS) Services
Part No Description
200101 CS Standard Sequencing
Include cycle sequencing reactions, dye terminator removal, capillary electrophoresis run and result analysis.

200103 CS Ready-to-run Sequencing
Include capillary electrophoresis run and result analysis only.

200104 CS Ready-to-run Sequencing with Dye Removal
Include dye terminator removal, capillary electrophoresis run and result analysis.

200105 CS PCR Clean-up Sequencing
Include unpurified PCR template clean-up by either gel or column methods, followed by Standard Sequencing service.

200106 CS Clone Sequencing
Include single colony growth, plasmid template extraction and purification, followed by Standard Sequencing service.

200107 CS Primer Walking Sequencing
Include subsequent primer design in one or both directional to fully sequence the region of interest in the plasmid.