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Other Services
The following services are provided :
microRNA Expression Service details:
The service includes:
- Phosphatase treatment of total RNA
- Labeling of microRNA with Cy-3-pCp
- Quality Control.
- Hybridization of labeled RNA to Agilent Microarray slide
- Scanning and Image Analysis
- Data Processing/Spot Analysis and Data Analysis report from Agilent Genespring GX (optional)
Guidelines for Sample Submission
- The miRNA Sample Submission Form must be duly completed and received by Research Biolabs, before shipping of samples.
- Customer shall provide 1 microgram of total RNA (concentration ≥ 50ng/ul) in non-DEPC-treated RNAse-free water, sent in nuclease-free screw-capped, 1.5ml microcentrifuge tubes. Shipment of samples should be in dry ice.
- Customer must submit proof of sample quality (electrophoresis gel image or Agilent Bioanalyzer readings) together with sample submission form, showing no signs of degradation or contamination.
- Samples must have OD readings of A260/A280 ≥1.7 and A260/A230 ≥1.5.
- Quality control procedures will be done after various steps during the experiment and the customer will be informed if the samples fail QC.
- Results will be delivered within 4 weeks of receipt of samples. Research Biolabs Pte Ltd shall not be responsible for delays due to shipments or lost/degraded samples during transportation.
- If complications arise in meeting with these requirements, please contact our Lab Manager for further advice at arrays@aitbiotech.com or call 65-6777 5366.
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Gene Expression Service details:
The service includes:
- Amplification of RNA using Agilent’s amplification kit
- Labeling with Cy-3, Cy-5
- Quality Control.
- Hybridization of labeled cRNA to Agilent Microarray slides
- Scanning and Image Analysis
- Data Processing and Data Analysis report from Agilent Genespring GX
Guidelines for Sample Submission
- The Gene Expression Sample Submission Form must be duly completed and received by Research Biolabs, before shipping of samples.
- Customer shall provide 1 microgram each of total RNA from treated/test sample and untreated/control sample (concentration ≥ 100ng/ul) in non-DEPC-treated RNAse-free water, sent in nuclease-free screw-capped, microcentrifuge tubes. Shipment of samples should be in dry ice.
- Customer must submit proof of sample quality (electrophoresis gel image or Agilent Bioanalyzer readings) together with sample submission form, showing no signs of degradation or contamination.
- Samples must have OD readings of A260/A280 ≥1.7 and A260/A230 ≥1.5.
- Quality control procedures will be done after various steps during the experiment and the customer will be informed if the samples fail QC.
- Results will be delivered within 4 weeks of receipt of samples. Research Biolabs Pte Ltd shall not be responsible for delays due to shipments or lost/degraded samples during transportation.
- If complications arise in meeting with these requirements, please contact our Lab Manager for further advice at arrays@aitbiotech.com or call 65-6777 5366
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ChIP-on-chip Service details:
The service includes:
- Labeling of DNA with Cy-3, Cy-5
- Quality control
- Hybridization of labeled DNA to Agilent Microarray slides
- Scanning and Image Analysis
- Data Processing and Data Analysis report from Agilent ChIP Analytics
Guidelines for Sample Submission
- The ChIP-on-chip Sample Submission Form must be duly completed and received by Research Biolabs, before shipping of samples.
- Customer shall provide 3 micrograms each of whole cell extract DNA and IP DNA that has been amplified and validated for enrichment of known regions, sent in nuclease-free screw-capped, microcentrifuge tubes (concentration ≥ 100ng/ul).
- Customer must submit proof of sample quality (electrophoresis gel image or Agilent Bioanalyzer readings) together with sample submission form, showing no signs of degradation or contamination.
- Samples must have OD readings of A260/A280 ≥1.7 and A260/A230 ≥1.5.
- Quality control procedures will be done after various steps during the experiment and the customer will be informed if the samples fail QC.
- Results will be delivered within 4 weeks of receipt of samples. Research Biolabs Pte Ltd shall not be responsible for delays due to shipments or lost/degraded samples during transportation.
- If complications arise in meeting with these requirements, please contact our Lab Manager for further advice at arrays@aitbiotech.com or call 65-6777 5366.
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Array CGH Service details:
The service includes:
- Labeling of DNA with Cy-3, Cy-5, using Agilent’s Genomic DNA Labeling kit
- Quality control
- Hybridization of labeled DNA to Agilent Microarray slides
- Scanning and Image Analysis
- Data Processing and Data Analysis report from Agilent CGH Analytics
Guidelines for Sample Submission
- The aCGH Sample Submission Form must be duly completed and received by Research Biolabs, before shipping of samples.
- Customer shall provide 2 micrograms each of test sample and reference sample (concentration ≥ 100ng/ul) genomic DNA (gDNA) in non-DEPC-treated RNAse-free water or TE and sent in nuclease-free screw-capped, microcentrifuge tubes. Alternatively, gDNA can be shipped lyophilized at room temperature; or in ethanol with 0.1 volume of sodium acetate and 2.5 volumes of ethanol (100%), shipped on ice.
- Customer must submit proof of sample quality (electrophoresis gel image or Agilent Bioanalyzer readings) together with sample submission form, showing no signs of degradation or contamination.
- Samples must have OD readings of A260/A280 ≥1.7 and A260/A230 ≥1.5.
- Quality control procedures will be done after various steps during the experiment and the customer will be informed if the samples fail QC.
- Results will be delivered within 4 weeks of receipt of samples. Research Biolabs Pte Ltd shall not be responsible for delays due to shipments or lost/degraded samples during transportation.
- If complications arise in meeting with these requirements, please contact our Lab Manager for further advice at arrays@aitbiotech.com or call 65-6777 5366.
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