DNA Cloning

We offer a service for cloning the gene of interest into the chosen vectors. Our experienced staff are able to advise on cloning strategies and will work with you to develop the protocol most suited to your needs. We have successfully completed many cloning projects involving a variety of systems and employ state of the art techniques throughout.

 

Type of service

• Subcloning

We will clone your insert from the source vector into a plasmid vector of your choice. After transformation, clones will be screened for the correct restriction fragments.

• PCR Cloning

Using a DNA sequence of your specification, we will design and synthesize primers for PCR/RT-PCR amplification of the desired sequence from your DNA or RNA source. The PCR product will be digested with restriction enzymes and cloned into the plasmid vector of your choice. High fidelity polymerase is used in PCR to minimize the amplification errors.

• TA cloning

Using a DNA sequence of your specification, we will design and synthesize primers for PCR/RT-PCR amplification of the desired sequence from your DNA or RNA source. The PCR product will be directly cloned into QiagenTM pDrive vector using the QIAGEN PCR CloningPlus Kit.

 

Verification:

Each vector construct generated is verified by restriction enzyme digest and DNA sequencing in both directions.

 

You will receive:

1. Glycerol stocks of verified clone;
2. At least 1.0 µg of the construct DNA*.
3. A detailed report including the experimental strategy, raw data and final results

* If large scale plasmid DNA purification is needed, please refer to our plasmid DNA preparation services.

 

Turnaround time:

2-4 weeks

 

Contact information

For further information, please contact sales@aitbiotechbiolabs.com or call us at (65) 67786822.

 

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